Coding

Part:BBa_K1702005:Design

Designed by: Olivia Smith, Adriana Collings   Group: iGEM15_CSU_Fort_Collins   (2015-09-17)


Trans-zeatin biosynthesis under control of the lac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 930
    Illegal AgeI site found at 1302
    Illegal AgeI site found at 1310
    Illegal AgeI site found at 1466
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The piece was synthesized with the two genes back-to-back (i.e. as an operon) and expression can be induced using lactose or IPTG.


Source

This piece was synthesized based on sequences from Kamada-Nobusada, Tomoe and Sakakibara, Hitoshi. "Molecular basis for cytokinin biosynthesis." Phytochemistry 70:444-449. and from KEGG (kegg.jp).

References